JKAOH Journal of Korean Acadomy of Oral Health
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Fig. 1. Effect of bile acids on the viability of RAW 264.7 cells. RAW 264.7 cells (3×103 cells/well) were seeded in 96 well-plates and treated with culture medium alone (control) or in combination with bile salts. After 24 hours, the cell viabilities represented as optical density at 570 nm were measured by MTT-assay. (A, B) Sodium taurodeoxycholate and sodium taurocholate were treated at the range of 0-1,000 µM. The cell viabilities of them were no significant differences with that of the control group. However, both groups presented cytotoxicity to the cells 500 and 1,000 µM (P<0.001). (C) Sodium glycocholate treated at the range of 0-4,000 µM presented no cytotoxic effect up to a maximal 4,000 µM. Results were expressed as the relative cell viabilities (%) compared to the control group (no bile salt; 0 µM) as a reference. Bar and error bar present the mean±S.D. of % cell viability relative to the control. ***P<0.001 significantly different from the control. Each point is repeatedly measured.
J Korean Acad Oral Health 2020;44:205~213 https://doi.org/10.11149/jkaoh.2020.44.4.205
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