JKAOH Journal of Korean Acadomy of Oral Health
pISSN 1225-388X eISSN 2093-7784

Download original image
Fig. 2. Effects of three bile acids on expression of pro-inflammatory cytokines in Pg-LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were cultured at a density of 3×104 cells/well in 6-well plates and treated with either culture medium alone (control) and three bile salts with the serial concentrations in the range of 0-100 µM or 0-1,000 µM under the stimulation of Pg-LPS (1 µg/ml) for 12 hours. The levels of pro-inflammatory cytokines IL-6 and TNF-α in the culture supernatants were measured by ELISA. The production of IL-6 (A-C) and TNF-α (D-F) increased significantly under Pg-LPS stimulation alone compared to the control, while the expression of them tended to be decreased with increasing treated concentration of three bile salts in a dose-dependent manner compared to only Pg-LPS group. (A-C) The production of IL-6 was strongly inhibited at concentrations equal to or higher than 100 µM of sodium taurodeoxycholate or 10 µM of sodium glycocholate treatment, each (P<0.001). (D-F) The production of TNF-α was suppressed as the concentration of three bile salts increased although the significance was relatively weak than IL-6. The levels of TNF-α were significantly lowered only in the treatment of sodium glycocholate at 1,000 µM (P<0.05). Results (bar and error bar) present the mean±S.D. of cytokine levels (pg/ml). *P<0.05 , **P<0.01, and ***P<0.001 mean significant differences from Pg-LPS only group. ‘a’ means no significant differences with the control (no Pg-LPS) group. Each point is repeatedly measured (n=6 well in treatment, n=3 in ELISA). Pg-LPS, Porphyromonas gingivalis lipopolysaccharide; IL-6, interleukin-6; TNF-α, tumor necrosis factor-a; ELISA, enzyme-linked immunosorbent assay.
J Korean Acad Oral Health 2020;44:205~213 https://doi.org/10.11149/jkaoh.2020.44.4.205
© Journal of Korean Academy of Oral Health