JKAOH Journal of Korean Acadomy of Oral Health
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Fig. 2. Investigation of apoptosis and necrosis. Nicotine-treated HGF for 24 h and 48 h was treated with 2 mM ethylenediaminetetraacetic acid in PBS to detach from cell culture dish. The cell was washed twice with cold PBS. The cells were reacted 1.25 µl of Annexin V-FITC in Binding buffer (200 µl; 0.1 M HEPES, pH7.4; 1.4 M NaCl; 25 mM CaCl2) for 15 min at RT in the dark and then add 4 µl of propidium iodine (0.05 mg/ml). The cells were analyzed by a fluorescence-activated cell sorter.
J Korean Acad Oral Health 2020;44:214~221 https://doi.org/10.11149/jkaoh.2020.44.4.214
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