JKAOH Journal of Korean Acadomy of Oral Health
pISSN 1225-388X eISSN 2093-7784

Table. 3.

Inhibitory effect of nicotine on cell proliferation

  MTT assay

1st 2nd 3rd Mean±SD
Control 0.13 0.15 0.14 0.14±0.01
FGF 0.22 0.22 0.23 0.22±0.00
FGF+nicotine 1 mM 0.17 0.14 0.15 0.15±0.01*
FGF+nicotine 10 mM 0.12 0.11 0.13 0.12±0.01*
FGF+nicotine 100 µM 0.11 0.10 0.11 0.11±0.00*

The gingival fibroblasts were cultured in DMEM medium containing 10% fetal bovine serum, and cells were dropped using trypsin-EDTA and dispensed into a 96-well polystyrene culture dish at 1×103 cells/ml at a concentration of 10 ng/ml was added to the culture medium, Of nicotine was added to the culture medium to remove the existing culture medium. After incubation in a 5% CO2 incubator for 12 hours, cell growth was examined using MTT. *Statistically significant difference compared with fibroblast growth factor (P<0.05).

FGF, fibroblast growth factor.

J Korean Acad Oral Health 2020;44:214~221 https://doi.org/10.11149/jkaoh.2020.44.4.214
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