Fig. 7. Inhibitory effect of nicotine on cell proliferation. The gingival fibroblasts were cultured in DMEM medium containing 10% fetal bovine serum, and cells were dropped using trypsin-EDTA and dispensed into a 96-well polystyrene culture dish at 1×103 cells/ml at a concentration of 10 ng/ml was added to the culture medium, Of nicotine was added to the culture medium to remove the existing culture medium. After incubation in a 5% CO2 incubator for 12 hours, cell growth was examined using MTT. *Statistically significant difference compared with fibroblast growth factor (P<0.05). FGF, fibroblast growth factor.
© Journal of Korean Academy of Oral Health
