JKAOH Journal of Korean Academy of Oral Health

ISSN(Print) 1225-388X ISSN(Online) 2093-7784

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Original Article

Journal of Korean Academy of Oral Health 2019; 43(2): 72-77

Published online June 30, 2019 https://doi.org/10.11149/jkaoh.2019.43.2.72

Copyright © Journal of Korean Academy of Oral Health.

Effect of apoptosis on G361 cells by Cimicifuga rhizoma extract

Byul Bo Ra Choi1, Gyoo Cheon Kim2, Jin Woo Hong3, Sang Rye Park4

1Feagle Co., Ltd. , 2Department of Oral Anatomy, School of Dentistry, 3Department of Korean Internal Medicine, Pusan National University, Yangsan, 4Department of Dental Hygiene, Kyungnam College of Information & Technology, Busan, Korea

Received: January 29, 2019; Revised: March 26, 2019; Accepted: April 11, 2019

Abstract

Objectives: To investigate whether the cytotoxic effect of Cimicifuga rhizoma extract is associated with cell death in the human keratinocyte (HaCaT) and human melanoma cell lines (G361). Methods: Apoptosis induced by Cimicifuga rhizoma extract was confirmed by water-soluble tetrazolium salts-1 (WST-1) assay, immunocytochemistry, and western blot. Additionally, the release of cytochrome c and apoptosis-inducing factor (AIF) was visualized by confocal laser scanning microscopy.Results: The results showed that Cimicifuga rhizoma extract significantly reduced the viability of G361 cells with half-maximal inhibitory concentration (IC 50) of 200 μg/ml, and the apoptotic process was found to occur via the activation of caspase-3 and caspase-9 pathways. Besides, the release of cytochrome c and AIF was also detected. Conclusions: This study suggests that Cimicifuga rhizoma extract causes apoptosis of human melanoma cells through the intrinsic apoptotic pathway.

Keywords: Apoptosis, Cimicifuga rhizoma, Melanoma