Journal of Korean Academy of Oral Health 2019; 43(2): 72-77
Published online June 30, 2019 https://doi.org/10.11149/jkaoh.2019.43.2.72
Copyright © Journal of Korean Academy of Oral Health.
Byul Bo Ra Choi1, Gyoo Cheon Kim2, Jin Woo Hong3, Sang Rye Park4
1Feagle Co., Ltd. , 2Department of Oral Anatomy, School of Dentistry, 3Department of Korean Internal Medicine, Pusan National University, Yangsan, 4Department of Dental Hygiene, Kyungnam College of Information & Technology, Busan, Korea
Objectives: To investigate whether the cytotoxic effect of Cimicifuga rhizoma extract is associated with cell death in the human keratinocyte (HaCaT) and human melanoma cell lines (G361). Methods: Apoptosis induced by Cimicifuga rhizoma extract was confirmed by water-soluble tetrazolium salts-1 (WST-1) assay, immunocytochemistry, and western blot. Additionally, the release of cytochrome c and apoptosis-inducing factor (AIF) was visualized by confocal laser scanning microscopy.Results: The results showed that Cimicifuga rhizoma extract significantly reduced the viability of G361 cells with half-maximal inhibitory concentration (IC 50) of 200 μg/ml, and the apoptotic process was found to occur via the activation of caspase-3 and caspase-9 pathways. Besides, the release of cytochrome c and AIF was also detected. Conclusions: This study suggests that Cimicifuga rhizoma extract causes apoptosis of human melanoma cells through the intrinsic apoptotic pathway.
Keywords: Apoptosis, Cimicifuga rhizoma, Melanoma
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