J Korean Acad Oral Health 2022; 46(4): 217-221
Published online December 31, 2022 https://doi.org/10.11149/jkaoh.2022.46.4.217
Copyright © Journal of Korean Academy of Oral Health.
Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University, Cheonan, Korea
Correspondence to:Dong-Heon Baek
Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan 31116, Korea
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objectives: The aim of this study is to investigate antimicrobial activity in isolated Streptococcus salivarius against Gram-positive bacteria related oral diseases.
Methods: S. salivarius was used in G2, G7, K12, and ATCC 7073 strains and tryptic soy broth supplemented with glucose was cultivated. Actinomyces israelii, Actinomyces viscosus, and Enterococcus faecalis were cultivated with brain heart infusion broth. Streptococcus mutans and Streptococcus sobrinus were maintained using tryptic soy broth. The antimicrobial activity of S. salivarius was performed by minimum inhibitory concentration using the spent culture medium.
Results: All S. salivarius have antimicrobial activity against Gram-positive bacteria in oral cavity. When comparing antimicrobial activity, S. salivarius G2 and G7 as isolated strain showed stronger antimicrobial activity against Gram-positive microbe than type K12 strain.
Conclusions: S. salivarius G2 and G7 have strong antimicrobial activity and may be prevent oral disease by Gram-positive bacteria in oral cavity.
Keywords: Antimicrobial activity, Gram-positive bacteria, Probiotics, Salivaricin, Streptococcus salivarius
The infectious diseases in oral cavity can be divided two legions that it is a gingival and dental disease, and these infectious diseases are related oral biofilm that exists multi-species1). The biofilm of a healthy person is multi-species balance with high percentage of commensal bacteria. However, when this balance is disrobed and certain bacteria increase, it leads to oral disease2).
In this study, we investigated antimicrobial activity of different strains of
The antibacterial activity of
The data was obtained through experiment of three times in duplicate and analyzed Kruskal-Wallis test and Mann-Whitney U test using IBM SPSS statistics Ver. 23 (IBM, Armonk, NY, USA).
The antimicrobial activity of
Next, in experiment of the susceptibility test of
When the SCM was investigated antimicrobial activity against
In antimicrobial experiment using the SCM against
The antimicrobial activity of
In this study,
Most probiotics with antimicrobial activity are
The antimicrobial activity of most isolated
In this study, isolated
Based on previous studies and the data,
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