JKAOH Journal of Korean Academy of Oral Health

ISSN(Print) 1225-388X ISSN(Online) 2093-7784


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Original Article

Journal of Korean Academy of Oral Health 2013; 37(4): 187-193

Published online December 30, 2013 https://doi.org/10.11149/jkaoh.2013.37.4.187

Copyright © Journal of Korean Academy of Oral Health.

Antimicrobial effect of slightly acidic electrolyzed water on oral microorganisms

Yeon-Kyung Park

Department of Dental Technology, Gimcheon University, Gimcheon, Korea

Received: September 27, 2013; Revised: December 23, 2013; Accepted: December 27, 2013


Objectives: The purpose of this study was to evaluate the antibacterial effect of slightly acidic electro-lyzed water (SAEW) for use as a mouth-rinse on seven oral pathogens.Methods: In this study, SAEW with a pH of 6.2, oxidation-reduction potential of 728-800 mV, and avail-able chlorine concentration of 30 mg/L was produced by electrolysis using an electrolysis device. The antimicrobial properties of SAEW against seven oral pathogens were determined by the agar diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and mouth rinsing tests.Results: SAEW did not demonstrate any antimicrobial activity (disc diffusion method) against the seven oral pathogens under study. However, SAEW showed potent antimicrobial activity with an MIC value of 0.0075-0.015 mg/mL and a MBC value of 0.015-0.03 mg/mL. Mouth rinsing with SAEW showed 99.9% bacterial inhibition.Conclusions: SAEW exhibited potent antimicrobial activity against all oral pathogens causing dental caries and periodontal disease. Therefore, the results of this study suggest that SAEW can be used as an effective mouth-rinse to prevent oral diseases and for oral hygiene management, with potential for commercial application.

Keywords: Antibacterial effect, Dental caries, Mouthwashes, Oral pathogens, Periodontal disease, Electrolyzed weak acid water