JKAOH Journal of Korean Academy of Oral Health

ISSN(Print) 1225-388X ISSN(Online) 2093-7784

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Original Article

Journal of Korean Academy of Oral Health 2020; 44(1): 13-19

Published online March 30, 2020 https://doi.org/10.11149/jkaoh.2020.44.1.13

Copyright © Journal of Korean Academy of Oral Health.

Antibacterial effect of different concentrations of Galla Chinensis extract on cariogenic bacteria in a biofilm model

Eun-Jeong Kim1, Bo-Hyoung Jin2

1Dental Research Institution, 2Department of Preventive & Social Dentistry, School of Dentistry, Seoul National University, Seoul, Korea

Received: February 19, 2020; Revised: March 7, 2020; Accepted: March 7, 2020

Abstract

Objectives: Galla Chinensis inhibited the adherence of planktonic oral bacteria and acid production by cariogenic bacteria. However, little is known about the relevant conditions of Galla Chinensis extract (GCE) exposure time and concentration and the effect of GCE on the structural and functional activity of cariogenic bacteria. The antibacterial effects of natural G. Chinensis extract on S. mutans, S. sanguinis, and S. oralis biofilms were evaluated in vitro.
Methods: Biofilms formed on glass surfaces were treated with different concentrations of GCE at different exposure times. The effects were assessed by examining the bactericidal activity, acidogenesis, minimum inhibitory concentration, and morphology.
Results: There was a statistically significant difference in the bacterial growth inhibition depending on the concentration of the GCE, with bacterial growth being inhibited as the concentration of GCE increased. A concentration of 1.0 mg/ml GCE had similar bactericidal effects against S. mutans and S. oralis biofilms to those produced by 2.0 mg/ml CHX. In the 1.0 mg/ml GCE group, incomplete septa were also observed in the outline of the cell wall, together with disruption of the cell membrane. In addition, there was also a slight exudation of the intracellular content from the bacteria in the 1.0 mg/ml GCE and 2 mg/ml CHX groups.
Conclusions: These results indicate that GCE inhibits the growth of S. mutans, S. sanguinis, and S. oralis with increasing concentrations. It alters the microstructure of S. mutans biofilms. These results suggest that GCE might be a useful anti-bacterial agent for preventing dental caries.

Keywords: Antibacterial effects, Biofilms, Galla Chinensis, Streptococcus mutans